3-DIMENSIONAL IMAGE-FORMATION IN CONFOCAL MICROSCOPY

被引：71
|
作者：
SHEPPARD, CJR [1 ]
COGSWELL, CJ [1 ]
机构：
[1] UNIV OREGON,DEPT BIOL,EUGENE,OR 97403
来源：
JOURNAL OF MICROSCOPY-OXFORD | 1990年 / 159卷
关键词：
confocal microscopy; imaging theory; laser microscopy; scanning microscopy; Three‐dimensional imaging;
D O I：
10.1111/j.1365-2818.1990.tb04774.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Three‐dimensional (3‐D) imaging in confocal microscopes is considered in terms of 3‐D transfer functions. This leads to an explanation of axial imaging properties. The axial response was observed in both object‐scanning and beam‐scanning microscopes and the influence of off‐axis examination investigated. By simple processing of multi‐detector signals, imaging in both the axial and transverse directions can be improved. 1990 Blackwell Science Ltd
引用
收藏
页码：179 / 194
页数：16
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