DEVELOPMENT OF XYLOSE-FERMENTING YEASTS FOR ETHANOL-PRODUCTION AT HIGH ACETIC-ACID CONCENTRATIONS

被引：7
|
作者：
MOHANDAS, DV [1 ]
WHELAN, DR [1 ]
PANCHAL, CJ [1 ]
机构：
[1] VETROGEN CORP, LONDON, ON N6L 1A8, CANADA
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 51-2卷
关键词：
XYLOSE FERMENTATION; ACETIC ACID TOLERANCE; ADAPTATION; ACID HYDROLYSATES; SPENT SULFITE LIQUOR;
D O I：
10.1007/BF02933434
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mutants resistant to comparatively high levels of acetic acid were isolated from the xylose-fermenting yeasts Candida shehatae and Pichia stipitis by adapting these cultures to increasing concentrations of acetic acid grown in shake-flask cultures. These mutants were tested for their ability to ferment xylose in presence of high acetic acid concentrations, in acid hydrolysates of wood, and in hardwood spent sulfite liquor, and compared with their wild-type counterparts and between themselves. The P. stipitis mutant exhibited faster fermentation times, better tolerance to acid hydrolysates, and tolerance to lower pH.
引用
收藏
页码：307 / 318
页数：12
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