COLUMN LIQUID-CHROMATOGRAPHY WITH DIODE LASER-INDUCED FLUORESCENCE DETECTION OF CARBOXYLIC-ACIDS AFTER PRECOLUMN DERIVATIZATION

被引：12

作者：

MANK, AJG ^{[1
]}

BEEKMAN, MC ^{[1
]}

VELTHORST, NH ^{[1
]}

BRINKMAN, UAT ^{[1
]}

LINGEMAN, H ^{[1
]}

GOOIJER, C ^{[1
]}

机构：

[1] FREE UNIV AMSTERDAM,DEPT GEN & ANALYT CHEM,1081 HV AMSTERDAM,NETHERLANDS

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 315卷 / 1-2期

关键词：

FLUOROMETRY; LASER-INDUCED FLUORESCENCE; RED-ABSORBING LABEL; CARBOXYLIC ACIDS; NAPROXEN;

D O I：

10.1016/0003-2670(95)00334-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Several pre-column approaches to fluorescence derivatisation of carboxylic acids, compatible with diode laser-induced fluorescence (DIO-LIF) detection, were compared. For this purpose red-absorbing labels containing hydrazide, bromoacetamide and iodoacetamide functionalities were studied. The sensitivity of DIO-LIF detection is illustrated by the (3-10) X 10-(12) M (75-250 amol) detection limit for various derivatised carboxylic acids. The real-life detection limit for the carboxylic acids is 3 x 10(-8) M, because derivatisation is not quantitative at lower concentrations. Derivatised n-alkylcarboxylic acids and non-steroidal anti-inflammatory drugs are easily separated by column liquid chromatography, despite the attachment of the bulky red-absorbing labels. The determination of the anti-inflammatory drug naproxen in saliva is shown as an example.

引用

页码：209 / 216

页数：8

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