Virus-induced gene complementation in tomato

被引：13
|
作者：
Kong, Junhua [1 ]
Chen, Weiwei [1 ]
Shen, Jiajia [1 ]
Qin, Cheng [1 ]
Lai, Tongfei [1 ]
Zhang, Pengcheng [1 ]
Wang, Ying [1 ]
Wu, Chaoqun [1 ]
Yang, Xin [2 ]
Hong, Yiguo [1 ,2 ]
机构：
[1] Hangzhou Normal Univ, Coll Life & Environm Sci, Res Ctr Plant RNA Signaling, Hangzhou, Zhejiang, Peoples R China
[2] Univ Warwick, Sch Life Sci, Coventry, W Midlands, England
来源：
PLANT SIGNALING & BEHAVIOR | 2013年 / 8卷 / 11期
基金：
中国国家自然科学基金; 英国生物技术与生命科学研究理事会;
关键词：
plant virus technology; functional complementation; VIGC; ripening inhibitor; colourless nonripening; Potato virus X;
D O I：
10.4161/psb.27142
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Virus-induced gene complementation (VIGC), a plant virus technology based on Potato virus X for transient overexpression of endogenous genes complemented tomato mutants, resulting in non-ripening fruits to ripen. This efficient "gain-of-function" approach involves no stable transformation, and reveals a fruit-specific transcriptional network that may exist among key transcription factors in modulating tomato ripening. Thus, VIGC represents a novel and feasible strategy for gene functional analysis in plants.
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页数：2
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