COMPARISON OF INDIRECT IMMUNOFLUORESCENCE ON CRITHIDIA-LUCILIAE, FARR ASSAY AND NEW ELISA TECHNIQUES FOR THE DETECTION OF ANTI-NATIVE DNA ANTIBODIES

被引：0

作者：

CHRETIEN, P ^{[1
]}

DAUVIN, M ^{[1
]}

HELIN, P ^{[1
]}

OCWIEJA, T ^{[1
]}

ABSALON, YB ^{[1
]}

JOHANET, C ^{[1
]}

机构：

[1] HOP ST ANTOINE,IMMUNOL LAB,F-75012 PARIS,FRANCE

来源：

ANNALES DE BIOLOGIE CLINIQUE | 1994年 / 52卷 / 09期

关键词：

AUTOANTIBODIES; DNA; FARR ASSAY; IMMUNOFLUORESCENCE TECHNIQUE; ELISA;

D O I：

暂无

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Anti-native DNA (ds DNA) antibodies were measured by the Farr assay, an indirect immunofluorescence on Crithidia luciliae (IIFCL) and a newly commercialised enzyme-linked immunosorbent assay (Elisa) in the sera of 270 patients: 73 negative controls, 67 patients with systemic lupus erythematosus (SLE), 73 with clinical symptoms of another connective tissue disease and 61 patients with liver diseases. The Farr assay remains a reliable method, IIFCL seems to be as specific but less sensitive and Elisa has a higher sensitivity but a lower specificity. Finally, 35 sera from the 270 patients were tested for anti-ds DNA antibodies using two other commercialised Elisa-related techniques. The results suggest little correlation between the three Elisa methods.

引用

页码：645 / 650

页数：6

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