S-ANTIGEN LOCALIZATION IN THE ERYTHROCYTIC STAGES OF PLASMODIUM-FALCIPARUM

被引：15
|
作者：
CULVENOR, JG
CREWTHER, PE
机构：
[1] Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria
来源：
JOURNAL OF PROTOZOOLOGY | 1990年 / 37卷 / 01期
关键词：
Human; immunoelectron microscopy. Malaria; parasitophorous vacuole; Plasmodium falciparum; S‐antigen;
D O I：
10.1111/j.1550-7408.1990.tb01117.x
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
Localization of the S‐antigen of Plasmodium falciparum isolate FCQ27/PNG, from Papua New Guinea, was studied by post‐embedding immunoelectron microscopy using affinity‐purified rabbit antibodies raised against the repeat region of the antigen. Labelling was found in the parasitophorous vacuole (PV) space of early to late schizonts and in PV‐related vesicles within the erythrocyte cytoplasm of schizont‐infected cells. Other subcellular structures within the erythrocyte cytoplasm were not labelled. After breakdown of the PV membrane, label was observed around the merozoites, consistent with mixing of the PV contents and erythrocyte cytoplasm. The antigen was not found in uninfected cells, ring stages, trophozoites or associated with free merozoites. Antibodies to FCQ27/PNG S‐antigen did not react with other isolates tested, whereas rabbit antibodies to the Palo Alto/Wellcome S‐antigen repeat region reacted with isolates FCR3 and ItG2F6 but not with FCQ27/PNG. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：59 / 65
页数：7
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