ACTIVATION OF EMBRYONIC GENES DURING PREIMPLANTATION RAT DEVELOPMENT

Expression of the embryonic genome has been examined during preimplantation rat development. Proteins synthesized at different stages of embryogenesis were labelled with [S-35]methionine and then separated by one-dimensional gel electrophoresis. A major transformation in the pattern of protein synthesis has been observed between the two- and the four-cell stages of embryonic development. Also the culture of embryos with an inhibitor of transcription (alpha-amanitin) has shown that the first alpha-amanitin-sensitive events take place during the late two-cell stage. However, inhibition of transcription does not arrest the embryo development up to the four-cell stage. Taken together, the results indicate that in rats the initiation of embryonic gene activation occurs at the late two-cell stage. However, the first two cleavage divisions can occur in the absence of transcription. (C) 1994 Wiley-Liss, Inc.