QUANTITATIVE-ANALYSIS OF CYTOKININS BY ELECTROSPRAY TANDEM MASS-SPECTROMETRY

被引：72
|
作者：
PRINSEN, E [1 ]
REDIG, P [1 ]
VANDONGEN, W [1 ]
ESMANS, EL [1 ]
VANONCKELEN, HA [1 ]
机构：
[1] UNIV ANTWERP,RUCA,DEPT CHEM,NUCLEOSIDE RES & MASS SPECTROMETRY UNIT,B-2020 ANTWERP,BELGIUM
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 10期
关键词：
D O I：
10.1002/rcm.1290091016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A methodology was developed for the analysis of cytokinins from plant tissue using electrospray tandem mass spectrometry in combination with HPLC. A detection limit of 1 pmol zeatin-riboside injected on-column was achieved under multiple reaction monitoring conditions. Linearity was observed within a concentration range of 1-100 pmol injected on-column. In view of the analysis of a considerable amount of samples, the chromatographic conditions were selected which were a compromise between speed of analysis and resolution, This approach, in combination with multiple reaction monitoring, allowed the analysis of up to 150 samples a day, and quantitation of 16 different cytokinins per sample.
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收藏
页码：948 / 953
页数：6
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