STREPTOMYCES-AUREOFACIENS STRAINS AS HOSTS FOR CLONING OF GENES AFFECTING ANTIBIOTIC PRODUCTION

被引：4
|
作者：
GODANY, A
NAZAROV, V
OKTAVCOVA, B
SEVCIKOVA, B
KOTERCOVA, I
机构：
[1] Institute of Molecular biology, Slovak Academy of Sciences, Bratislava, 842 51
来源：
BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 07期
关键词：
D O I：
10.1007/BF01049202
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Several mutants of Streptomyces aureofaciens strain were used for protoplast regeneration and plasmid transformation. All tested mutants (excepting R 8/26) were transformable by number of plasmids and shuttle vectors. The transformation of the CTC production strains by plasmid containing cloned CTC resistance gene resulted in 1, 1-4 times higher antibiotic production. From the restriction analysis of plasmid, phage and chromosomal DNAs it vas estimated, that all tested mutants normally contain the modification system analogous to Nae I (Roberts, 1987). Mutant R 8/26 expresses not only complete restriction-modification system mentioned above but also potential second system restricting several actinophages.
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页码：471 / 476
页数：6
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