EXPRESSION OF ERYTHROID-DIFFERENTIATION FACTOR (EDF) IN CHINESE-HAMSTER OVARY CELLS

被引：55

作者：

MURATA, M ^{[1
]}

ONOMICHI, K ^{[1
]}

ETO, Y ^{[1
]}

SHIBAI, H ^{[1
]}

MURAMATSU, M ^{[1
]}

机构：

[1] UNIV TOKYO, FAC MED, DEPT BIOCHEM, BUNKYO KU, TOKYO 113, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1988年 / 151卷 / 01期

关键词：

D O I：

10.1016/0006-291X(88)90583-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasmid DNA containing EDF subunit cDNA and mouse dihydrofolate reductase (DHFR) cDNA was transfected into CHO DHFR- cells by the calcium-phosphate method. DHFR positive transformants secreted recombinant EDF (r-EDF) constitutively in an active form and accumulated it in the conditioned medium. Furthermore, cells which were resistant to methotrexate (MTX: 0.5 .mu.M) secreted r-EDF up to 1 .mu.g/ml. r-EDF was identical to natural EDF (n-EDF) produced by human acute monocytic leukemia cell line, THP-1, as regards its dimeric structure and a biological activity.

引用

页码：230 / 235

页数：6

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