SURVEY OF EXTRA-INTESTINAL IMMUNE RESPONSES IN ASYMPTOMATIC LONG-TERM CAMPYLOBACTER JEJUNI-INFECTED MICE

被引:39
|
作者
Heimesaat, Markus M. [1 ]
Haag, Lea-Maxie [1 ]
Fischer, Andre [1 ]
Otto, Bettina [1 ]
Kuehl, Anja A. [2 ,3 ]
Goebel, Ulf B. [1 ]
Bereswill, Stefan [1 ]
机构
[1] Univ Med Berlin, Dept Microbiol & Hyg, Charite, Centrum 5,Campus Benjamin Franklin,Hindenburgdam, Berlin, Germany
[2] Univ Med Berlin, Dept Gastroenterol Infectiol & Rheumatol, Charite, Berlin, Germany
[3] Charite Univ Med Berlin, Res Ctr Immunosci RCIS, Berlin, Germany
来源
EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY | 2013年 / 3卷 / 03期
关键词
Campylobacter jejuni; infant mice; long-term C. jejuni infection; intestinal microbiota; colonization resistance; host-pathogen interaction; innate and adaptive immunity; extra-intestinal immune cell influx; proliferative cells; apoptotic cells; macrophages; T lymphocytes; B lymphocytes;
D O I
10.1556/EuJMI.3.2013.3.4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Campylobacter jejuni is among the most frequently reported bacterial pathogens causing diarrhea in humans worldwide. We recently reported a murine infection model mimicking key features of human campylobacteriosis. Six days following oral C. jejuni infection immediately after weaning, infant mice developed acute enterocolitis resolving within 2 weeks. Thereafter, C. jejuni could still be isolated from the intestines of asymptomatic mice at low levels accompanied by distinct immune responses, both at intestinal and extra-intestinal locations. We here show that, at day 103 post infection (p.i.), long-term C. jejuni-infected mice exhibited higher numbers of T lymphocytes in liver, lung, kindneys, and cardiac muscle as compared to uninfected controls. In addition, B lymphocytes were slightly higher, but macrophage numbers were significantly lower in liver and lung of C. jejuni-infected versus naive mice. As compared to uninfected control animals, proliferating cells were significantly lower in liver, lung, kidneys, cardiac muscle, and spleen at day 103 p.i., whereas more apoptotic cells were abundant in the spleen with predominance in the red pulp. This study underlines that post-infectious, immunological sequelae at extra-intestinal locations are of importance even in asymptomatic long-term C. jejuni carriers and need to be further studied in order to unravel the underlying molecular mechanisms.
引用
收藏
页码:174 / 182
页数:9
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