CHARACTERIZATION OF THE GLYCOPROTEIN-D GENE-PRODUCTS OF EQUINE HERPESVIRUS-1 USING A PROKARYOTIC CELL EXPRESSION VECTOR

被引：20
|
作者：
LOVE, DN [1 ]
BELL, CW [1 ]
WHALLEY, JM [1 ]
机构：
[1] MACQUARIE UNIV,SCH BIOL SCI,SYDNEY,NSW 2109,AUSTRALIA
来源：
VETERINARY MICROBIOLOGY | 1992年 / 30卷 / 04期
关键词：
D O I：
10.1016/0378-1135(92)90024-N
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The gene encoding equine herpesvirus 1 (equine abortion virus; EHV-1) glycoprotein D was engineered into the prokaryotic vector pEX, and expressed as a beta-galactosidase fusion product, which was recognized by pooled equine sera and anti-EHV-1 rabbit sera. Antibodies raised against the EHV-1 gD fusion product identified strong bands in infected cells at 66 and 68 K and at 138 K in purified virus, thus characterizing the several forms of this major envelope glycoprotein which is an important candidate for inclusion in subunit vaccines.
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页码：387 / 394
页数：8
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