DETERMINATION OF MAGNESIUM-ION BY USE OF THE COUPLED-ENZYME METHOD WITH PYRUVATE-KINASE AND LACTATE-DEHYDROGENASE

被引：0
|
作者：
SHIMOFURUYA, H
NAKAMURA, M
SUZUKI, J
机构：
[1] TOYOHASHI UNIV TECHNOL,SCH MAT SCI ENGN,TOYOHASHI,AICHI 441,JAPAN
[2] TOYOHASHI UNIV TECHNOL,DEPT ELECT ENGN,TOYOHASHI,AICHI 441,JAPAN
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1995年 / 35卷 / 04期
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D O I：
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中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a new convenient method for determination of magnesium ion concentrations by use of magnesium ion-dependent enzymes; pyruvate kinase and lactate dehydrogenase. This method is based on the determination of the reaction rate of pyruvate kinase which uses MgADP(-) as substrate. The reaction rate of pyruvate kinase is dependent upon the formation of the complex with ADP and magnesium ion and the amount of the complex is dependent upon that of magnesium ion. The reaction rate of pyruvate kinase can be easily and spectrophotometrically determined by using lactate dehydrogenase and NADH as the decreased amount of absorbance at 350 nm.
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页码：757 / 762
页数：6
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