DETERMINATION OF CLODRONATE IN AQUEOUS-SOLUTIONS BY HPLC USING POSTCOLUMN DERIVATIZATION

被引：15
|
作者：
VIRTANEN, V
LAJUNEN, LHJ
机构：
[1] Department of Chemistry, University of Oulu, SF-90570 Oulu, Linnanmaa
来源：
TALANTA | 1993年 / 40卷 / 05期
关键词：
D O I：
10.1016/0039-9140(93)80275-V
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for the determination of clodronate at ppm levels in aqueous solutions by HPLC is presented. Clodronate is first separated by anion exchange chromatography using a solution of nitric acid and sodium nitrate as eluent. Th-EDTA-XO mixed ligand complex is used as a postcolumn reagent for clodronate. Clodronate reacts quantitatively with this complex in slightly acid solutions, and a change in the absorbance of Th-EDTA-XO is used as a measure for the clodronate concentration. The detection limit is about 3 ppm, and the linear dynamic range of the method extends up to about 200 ppm. The RSD values varied between 5 and 16%. The effect of pH on appearance and retention of different forms of clodronate is studied. Different forms of clodronate can be separated and detected in the used anion-exchange system.
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页码：661 / 667
页数：7
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