HYPOTONIC TREATMENT LEADS TO CHROMOSOMAL-ABERRATIONS BUT NOT TO SISTER-CHROMATID EXCHANGES IN HUMAN-LYMPHOCYTES

被引：12
|
作者：
KALWEIT, S
NOWAK, C
OBE, G
机构：
[1] UNIV ESSEN GESAMTHSCH,FACHBEREICH 9,DEPT GENET,POB 103764,W-4300 ESSEN 1,GERMANY
[2] FREE UNIV BERLIN,INST GENET,W-1000 BERLIN 33,GERMANY
来源：
MUTATION RESEARCH | 1990年 / 245卷 / 01期
关键词：
Chromosome aberration; Human lymphocytes; Hypotonic treatment;
D O I：
10.1016/0165-7992(90)90017-E
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Human peripheral lymphocytes were isolated from whole blood and exposed to culture medium of reduced osmolality. This hypotonic treatment led to a significant increase in the frequencies of chromosomal aberrations when the osmolality was reduced to 60 mOsm/kg H20 and below. Maximum damage occurred when the hypotonic treatment was done 27 or 30 h after starting the cultures. We also looked for the induction of sister-chromatid exchanges (SCE) by hypotonic culture conditions, but the SCE frequencies were not influenced. © 1990.
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页码：5 / 9
页数：5
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