HUMAN LIVER PROTEIN MAP - A REFERENCE DATABASE ESTABLISHED BY MICROSEQUENCING AND GEL COMPARISON

被引：127

作者：

HOCHSTRASSER, DF

FRUTIGER, S

PAQUET, N

BAIROCH, A

RAVIER, F

PASQUALI, C

SANCHEZ, JC

TISSOT, JD

BJELLQVIST, B

VARGAS, R

APPEL, RD

HUGHES, GJ

机构：

[1] UNIV GENEVA, DEPT MED BIOCHEM, CTR MED UNIV, CH-1211 GENEVA 4, SWITZERLAND

[2] UNIV GENEVA, MED DEPT & MED COMP, CH-1211 GENEVA 4, SWITZERLAND

[3] RED CROSS TRANSFUS CTR, LAUSANNE, SWITZERLAND

来源：

ELECTROPHORESIS | 1992年 / 13卷 / 12期

关键词：

D O I：

10.1002/elps.11501301201

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This publication establishes a reference human liver protein map obtained with immobilized pH gradients. By microsequencing, 57 spots or 42 polypeptide chains were identified. By protein map comparison and matching (liver, red blood cell and plasma sample maps), 8 additional proteins were identified. The new polypeptides and previously known proteins are listed in a table and/or labeled on the protein map, thus providing a human liver two-dimensional gel database. This reference map can be used to identify protein spots on other samples such as rectal cancer biopsies.

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页码：992 / 1001

页数：10

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