Kilohertz two-photon brain imaging in awake mice

被引：0

作者：

Tong Zhang

Oscar Hernandez

Radosław Chrapkiewicz

Adam Shai

Mark J. Wagner

Yanping Zhang

Cheng-Hsun Wu

Jin Zhong Li

Masatoshi Inoue

Yiyang Gong

Biafra Ahanonu

Hongkui Zeng

Haruhiko Bito

Mark J. Schnitzer

机构：

[1] Stanford University,James H. Clark Center

[2] Stanford University,CNC Program

[3] Stanford University,Howard Hughes Medical Institute

[4] The University of Tokyo,Department of Neurochemistry, Graduate School of Medicine

[5] Allen Institute for Brain Science,Department of Pathogenic Biology, College of Basic Medicine

[6] Jilin University,Department of Bioengineering

[7] Stanford University School of Medicine,Department of Biomedical Engineering

[8] Duke University,undefined

来源：

Nature Methods | 2019年 / 16卷

关键词：

D O I：

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中图分类号：

学科分类号：

摘要：

Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10–30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000–211,000 µm2 areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca2+ spiking with millisecond-scale timing resolution in the brains of awake mice.

引用

页码：1119 / 1122

页数：3

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