Facile immobilization of nitrile hydratase in SBA-15 via a biomimetic coating

被引：0

作者：

Yan Chen

Yanjun Jiang

Junkai Gao

Wenfeng Wu

Liangxiong Dong

Zejun Yang

机构：

[1] Zhejiang Ocean University,School of Port and Transportation Engineering

[2] Hebei University of Technology,School of Chemical Engineering and Technology

来源：

Journal of Porous Materials | 2017年 / 24卷

关键词：

Polyopamine; Biomimetic coating; Facile immobilization; Nitrile hydratase;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Dopamine functionalized mesoporous molecular sieve SBA-15 (Dop-SBA-15), synthesized by a simple and cost-effective method, was developed as a matrix to immobilize NHase (NHase@Dop-SBA-15). Various characterization techniques such as SEM, TEM, BET and FT-IR were carried out to investigate the properties of Dop-SBA-15 and NHase@Dop-SBA-15. It was shown that the Dop-SBA-15 had highly ordered mesopores and large surface area of 447 m2/g. The activity, thermal stability, pH stability, storage stability, and reusability of NHase@Dop-SBA-15 were studied in detail. It was observed that the performance of NHase@Dop-SBA-15 was improved significantly compared to that of the free NHase and NHase immobilized in unfunctionalized SBA-15. This study provided insight into the preparation of Dop-SBA-15 and its application for NHase immobilization.

引用

页码：787 / 793

页数：6

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