On–off switching of cell cycle and melanogenesis regulation of melanocytes by non-thermal atmospheric pressure plasma-activated medium

被引:0
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作者
Jin-Woo Lee
Se Jik Han
Hye Young Kang
Sung-Suk Wi
Min-Hyung Jung
Kyung Sook Kim
机构
[1] Kyung Hee University Medical Center,Medical Science Research Institute
[2] Graduate school,Department of Biomedical Engineering
[3] Kyung Hee University,Department of Biomedical Engineering
[4] College of Medicine,Department of Obstetrics & Gynecology
[5] Kyung Hee University,Healthcare Industry Research Institute
[6] Research Laboratory,undefined
[7] Medipl Co.,undefined
[8] Ltd,undefined
[9] School of Medicine,undefined
[10] Kyung Hee University,undefined
[11] Kyung Hee Medical Center,undefined
[12] Kyung Hee University,undefined
来源
Scientific Reports | / 9卷
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摘要
Non-thermal atmospheric pressure (NAP) plasma has demonstrated potential in biomedical applications, such as cancer treatment, bactericidal sterilization, and cell growth promotion or inhibition. In this study, for the first time, we demonstrated on–off switching of cell cycle progression and regulated melanogenesis in normal human skin melanocytes by NAP plasma-activated medium (PAM). The melanocytes were exposed to NAP plasma at durations varying from 0 to 20 min, and the effects of PAM on cell proliferation, cell cycle progression, and melanogenesis were investigated. Although PAM showed no cytotoxicity, the proliferation of melanocytes was inhibited. The melanocyte cell cycle was arrested by PAM for a relatively short period (48 h), after which it recovered slowly. PAM promoted melanogenesis through the activation of the enzymes tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2. These effects seem to be related to reactive oxygen species induced by PAM. Our finding that PAM modulates the cell cycle may provide insight into the recurrence of cancer. The regulation of the melanogenesis of melanocytes may facilitate the control of skin tone without incurring negative side effects.
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