Migratory activity of human glioma cell lines in vitro assessed by continuous single cell observation

被引：0
|
作者：
Tim Demuth
Nikolai J. Hopf
Oliver Kempski
Dieter Sauner
Monika Herr
Alf Giese
Axel Perneczky
机构：
[1] Department of Neurosurgery,Institute of Neuroradiology
[2] Neurooncology Laboratory,Department of Neurosurgery
[3] Institute of Neurosurgical Pathophysiology,undefined
[4] University Hospital,undefined
[5] University Hospital,undefined
[6] Eppendorf,undefined
来源：
Clinical & Experimental Metastasis | 2000年 / 18卷
关键词：
brain tumor; cell culture; glioma; invasion; migration; videomicroscopy;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A new migration assay, the time-lapse individual cell migration assay (TIM-assay), was developed, which allows the observation of cells over 24 h under controlled conditions. Using this technique, the migratory behavior of 8 human glioblastoma cell lines in vitro was studied. Special features are simultaneous documentation of migratory parameters of individual cells, i.e., migration velocities and migration paths of individual cells. Migration velocity for cell populations of the same cell line ranged from 0 to 24 μm/h. The migration paths were examined for being directional. Two thirds of all cells showed directional migration. Migration paths were further classified according to visual judgements for being linear, oscillating or mixed. The migration index had a mean of 91%. The presented TIM-assay allows the assessment of several new parameters, that may be useful to identify subgroups of gliomas with different biological characteristics.
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页码：589 / 597
页数：8
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