Adaptation responses of microalgal-bacterial granular sludge to polystyrene microplastic particles in municipal wastewater

Microplastics are frequently detected in wastewater treatment plants, but the knowledge of their effects on microalgal-bacterial granular sludge (MBGS) is still unknown. This study investigated the performance and adaptive response of MBGS exposed in municipal wastewater in the presence of polystyrene (PS) microplastic particles with different sizes (i.e., 100 nm, 5 μm, and 10 μm). Results indicated that the average removal efficiency of influent organics, ammonia, and phosphorus by MBGS process was stable at above 85%, showing insignificant difference between three sizes of microplastic particles. The community richness of MBGS was reduced by nano-sized (i.e., 100 nm) and micro-sized (i.e., 5 μm) PS microplastic particles, while the community diversity decreased in all types. Although filamentous cyanobacteria were broken by PS microplastic particles, the performance of MBGS process was insignificantly affected due to the stimulated extracellular polymeric substances, which could act as adaptive responses and protect MBGS from stress damage. This study proves that MBGS process can be operated in the presence of prevalent PS microplastic particles.