Effect of media on spore yield and thermal resistance of Bacillus stearothermophilus

被引：0
|
作者：
Thereza Christina Vessoni Penna
Irene A. Machoshvili
Marina Ishii
机构：
[1] University of São Paulo,Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science
[2] Rua Antonio de Macedo Soares,undefined
来源：
Applied Biochemistry and Biotechnology | 2003年 / 106卷 / 1-3期
关键词：
thermoresistance; sporulation; bioindicator;
D O I：
10.1385/ABAB:106:1-3:287
中图分类号：
学科分类号：
摘要：
The interference of eight components in the yield of sporulation and thermal resistance to moist heat (121°C) of Bacillus stearothermophilus spores suspended in 0.02 M calcium acetate solution and inoculated on paper strips previously treated with calcium acetate/calcium hydroxide was studied. The spore yield of 1.0×108/mL was developed at 62°C in 17 media containing different concentrations of d-glucose, sodium chloride, l-glutamic acid, yeast extract, peptone, manganese sulfate, potassium phosphate, and ammonium phosphate. The combined effects of yeast extract, peptone, and glucose contributed positively to the spore yield and to the stability of the thermal resistance of both spores in suspension and on strips.
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页码：287 / 294
页数：7
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