Effect of Bioactive Peptide Complex Isolated from Bovine Serum on Proliferation and Migration of Mesenchymal Stromal Cells In Vitro and Reparation of Bone Defects In Vivo

被引：0
|
作者：
A. I. Shaikhaliev
M. S. Krasnov
I. V. Vakhrushev
A. P. Il’ina
E. Yu. Rybakova
K. N. Yarygina
V. P. Yamskova
I. A. Yamskov
机构：
[1] Ministry of Health of the Russian Federation (Sechenov University),I. M. Sechenov First Moscow State Medical University
[2] Russian Academy of Sciences,A. N. Nesmeyanov Institute of Organoelement Compounds
[3] V. N. Orekhovich Research Institute of Biomedical Chemistry,N. K. Koltsov Institute of Developmental Biology
[4] Russian Academy of Sciences,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2019年 / 168卷
关键词：
bioregulator; blood serum; regenerative medicine; mesenchymal stromal cells; tooth pulp;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We studied the effect of a bioactive peptide complex isolated from bovine serum on the proliferative potential and migration rate of mesenchymal stromal cells in vitro, as well as on the healing of modeled bone defects in rats. This bioregulatory preparation stimulated proliferation of mesenchymal stromal cells from deciduous tooth pulp in vitro, but did not affect the rate of their migration in two-dimensional cultures. In vivo experiments showed that application of this preparation in combination with hydroxyapatite and chitosan gel accelerated bone tissue regeneration, thus ensuring restoration of morphologically normal bone matrix. Thus, cattle blood serum is an available source for the production of bioregulatory preparations for medical purposes.
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页码：178 / 185
页数：7
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