Rapid identification of Giardia duodenalis by loop-mediated isothermal amplification (LAMP) from faecal and environmental samples and comparative findings by PCR and real-time PCR methods

被引:0
|
作者
J. Plutzer
P. Karanis
机构
[1] Obihiro University of Agriculture and Veterinary Medicine,National Research Center for Protozoan Diseases
[2] National Institute of Environmental Health,Department of Water Hygiene
[3] University of Cologne,Molecular and Medical Parasitology, Institute II, Center of Anatomy
来源
Parasitology Research | 2009年 / 104卷
关键词
Lamp Assay; Qiagen GmbH; Lamp Reaction; Sewage Sample; Giardia Cyst;
D O I
暂无
中图分类号
学科分类号
摘要
The detection of two human-pathogenic Giardia duodenalis assemblages A and B in faecal and water samples by loop-mediated isothermal amplification (LAMP) has been evaluated. The LAMP reaction is reproducible, rapid and specific for the detection G. duodenalis and has lower costs compared to the other molecular assays. This is the first application of LAMP for Giardia detection.
引用
收藏
页码:1527 / 1533
页数:6
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