Urease immobilized on nylon: preparation and properties

被引：0
|
作者：
A. Anita
C. A. Sastry
M. A. Hashim
机构：
[1] Institute of Advanced Studies University of Malaya 50603 Kuala Lumpur,
来源：
Bioprocess Engineering | 1997年 / 17卷
关键词：
Enzyme; Thermal Stability; High Activity; Nylon; Glutaraldehyde;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Urease (EC 3.5.1.5) was covalently attached through glutaraldehyde to partially hydrolysed nylon 6/6 tubes. The highest activity of immobilized enzyme was obtained at 65 °C and pH 6.5, while the optimum temperature for free urease was found to be 25 °C. Immobilized urease showed an improved thermal stability in comparison to free urease. It retained 76% of the original activity after 60 days when stored at 4 °C and 78% of the activity after 5 repeated uses.
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页码：241 / 245
页数：4
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