Visualization of Reinke’s crystals in normal and cryptorchid testis

被引:0
|
作者
Viviana Kozina
David Geist
Lucie Kubinová
Ernest Bilić
Hans Peter Karnthaler
Thomas Waitz
Jiří Janáček
Oleksandr Chernyavskiy
Ivan Krhen
Davor Ježek
机构
[1] University of Zagreb,Department of Histology and Embryology, School of Medicine
[2] University of Vienna,Institute of Materials Physics
[3] Academy of Sciences of the Czech Republic,Department of Biomathematics, Institute of Physiology
[4] University of Zagreb,School of Medicine, Clinic for Pediatrics, University Hospital Centre “Zagreb”
[5] University of Zagreb,School of Medicine, Clinic for Urology, University Hospital Centre “Zagreb”
来源
Histochemistry and Cell Biology | 2011年 / 135卷
关键词
Testis; Reinke’s crystals; Cryptorchidism; Confocal microscopy; Electron microscopy; Stereology;
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学科分类号
摘要
Within the human testis, Reinke’s crystals are found in Leydig cells but their nature and function are poorly understood. The aim of our study was to investigate the properties of Reinke’s crystals in man with the normal morphology of the testis (control group) and infertile patients diagnosed with cryptorchidism. 20 biopsies from infertile patients and six biopsies from men with regular spermatogenesis (20–30 years.) were used. Sections of the testis tissue were stained with haematoxylin and eosin and a modified Masson’s method. Specimens were observed by bright field, confocal and transmission electron microscopy (TEM). The number of Reinke’s crystals in investigated groups was determined applying stereological methods. In both groups, Reinke’s crystals were noted within the cytoplasm and nuclei of Leydig cells. Some “free” crystals were found within the interstitial space, outside Leydig cells. Confocal microscopy proved to be very useful in the assessment of the shape and 3D reconstruction of the crystal. TEM analysis confirmed a hexagonal form of the crystal, while crystallographic data on sections of 70–300 nm thickness provided a better insight into the organization of the crystal lattice. Stereological analysis revealed a significant increase in the number of crystals in cryptorchid testes when compared with controls. Increased number of crystals in cryptorchid specimens leads to the assumption that the prolonged exposure to higher (abdominal) temperature might stimulate enzymes involved in the synthesis of the proteins of the crystal. However, the exact molecular nature of the crystal lattice remains in both normal and cryptorchid testis obscure.
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页码:215 / 228
页数:13
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