Validated RP-HPLC Method Development for Gentamicin Microanalysis in Pharmaceutical Dosage Forms, Urine and Blood

被引:0
|
作者
M. L. Maheshwari
N. Memon
A. A. Memon
M. Y. Khuhawer
A. H. Memon
机构
[1] University of Sindh,National Center of Excellence in Analytical Chemistry
[2] University of Sindh,Department of Pharmaceutical Chemistry, Faculty of Pharmacy
[3] University of Sindh,Institute of Advanced Research Studies in Chemical Sciences
[4] University of Sindh,Department of Pharmacognosy, Faculty of Pharmacy
来源
关键词
Gentamicin; Hantzsch reagent; derivatization; HPLC-PDA;
D O I
暂无
中图分类号
学科分类号
摘要
Gentamicin is an important member of the aminoglycoside class of antibiotics having broader spectrum with extend to ototoxicity and nephrotoxcity. A reliable and accurate high performance liquid chromatography (HPLC) procedure was established using Agilent Zorbax Eclipse plus phenyl hexyl C18 (4.6 × 250 mm, 5 μm) column and UV detection at 330 nm. Recovery of gentamicin from biological and pharmaceutical samples was more than 95-98% after spiking the drug. The sensitivity of method is predicted by calculating a lower level of the limit of detection and lower limit of quantification (0.286 and 0.867 μg/mL, respectively. The procedure was used to quantify gentamicin in biological fluids and pharmaceuticals dosage form.
引用
收藏
页码:524 / 530
页数:6
相关论文
共 50 条
  • [21] Development and Validation of Stability Indicating RP-HPLC Method for the Determination of Ezetimibe in Pharmaceutical Dosage Forms
    Nakarani, Mahendra P.
    Patel, Priyal R.
    Patel, Jayavadan K.
    Vaghani, Subhash S.
    LATIN AMERICAN JOURNAL OF PHARMACY, 2011, 30 (05): : 896 - 901
  • [22] STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF CHOLINE FENOFIBRATE IN PHARMACEUTICAL DOSAGE FORMS BY RP-HPLC
    Samyukta, E.
    Srinivas, Prathima
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2014, 5 (03): : 849 - 854
  • [23] Development and validation of RP-HPLC method for the estimation of agomelatine in bulk drug and pharmaceutical dosage forms
    Harika, B.
    Srikalyani, V.
    Nalluri, Buchi N.
    Vijaya Lakshmi, M.
    Journal of Chemical and Pharmaceutical Research, 2013, 5 (12) : 1162 - 1167
  • [24] A RP-HPLC method development and validation for the estimation of aliskiren hemifumarate in bulk and pharmaceutical dosage forms
    M.R. College of Pharmacy, Phool-Baugh, Vizianagaram, A.P, India
    不详
    不详
    不详
    Raul, S. K., 1600, Journal of Chemical and Pharmaceutical Research, 3/668 Malviya Nagar, Jaipur, Rajasthan, India (04):
  • [25] DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF RITONAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS
    Chiranjeevi, K.
    Channabasavaraj, K. P.
    Lakshminarayana, B.
    Kumar, B. Kalyan
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2011, 2 (02): : 336 - 340
  • [26] STABILITY INDICATING ASSAY METHOD DEVELOPMENT AND VALIDATION OF PREGABALIN IN PHARMACEUTICAL DOSAGE FORMS BY RP-HPLC
    Sneha, P.
    Srinivas, Prathima
    INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2015, 2 (06): : 1038 - 1047
  • [27] DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF RITONAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS
    Chiranjeevi, K.
    Channabasavaraj, K. P.
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2011, 2 (03): : 596 - 600
  • [28] RP-HPLC METHOD DEVELOPMENT AND VALIDTION FOR SIMULTANEOUS ESTIMATION OF TELMISARTAN AND RAMIPRIL IN PHARMACEUTICAL DOSAGE FORMS
    Divya, M.
    Anusha, G.
    Padmalatha, H.
    INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2016, 3 (02): : 86 - 91
  • [29] Development and Validation of RP-HPLC Method for the Estimation of Gemifloxacin Mesylate in Bulk and Pharmaceutical Dosage Forms
    Mohammad, Yunoos
    Kumar, B. Pragati
    Hussain, Azmath
    Harish
    E-JOURNAL OF CHEMISTRY, 2010, 7 (04) : 1621 - 1627
  • [30] A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE AND LISINOPRIL IN PHARMACEUTICAL DOSAGE FORM
    Chauhan, Vijendra
    Prajapati, Shailesh T.
    Patel, Chhaganbhai N.
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2011, 2 (07): : 1712 - 1715