Validated RP-HPLC Method Development for Gentamicin Microanalysis in Pharmaceutical Dosage Forms, Urine and Blood

被引：0
|
作者：
M. L. Maheshwari
N. Memon
A. A. Memon
M. Y. Khuhawer
A. H. Memon
机构：
[1] University of Sindh,National Center of Excellence in Analytical Chemistry
[2] University of Sindh,Department of Pharmaceutical Chemistry, Faculty of Pharmacy
[3] University of Sindh,Institute of Advanced Research Studies in Chemical Sciences
[4] University of Sindh,Department of Pharmacognosy, Faculty of Pharmacy
来源：
Pharmaceutical Chemistry Journal | 2021年 / 55卷
关键词：
Gentamicin; Hantzsch reagent; derivatization; HPLC-PDA;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Gentamicin is an important member of the aminoglycoside class of antibiotics having broader spectrum with extend to ototoxicity and nephrotoxcity. A reliable and accurate high performance liquid chromatography (HPLC) procedure was established using Agilent Zorbax Eclipse plus phenyl hexyl C18 (4.6 × 250 mm, 5 μm) column and UV detection at 330 nm. Recovery of gentamicin from biological and pharmaceutical samples was more than 95-98% after spiking the drug. The sensitivity of method is predicted by calculating a lower level of the limit of detection and lower limit of quantification (0.286 and 0.867 μg/mL, respectively. The procedure was used to quantify gentamicin in biological fluids and pharmaceuticals dosage form.
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页码：524 / 530
页数：6
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