Universal primers for the PCR-mediated amplification of DNA βA molecule associated with some monopartite begomoviruses

被引：0
|
作者：
R. W. Briddon
S. E. Bull
S. Mansoor
I. Amin
P. G. Markham
机构：
[1] John Innes Centre,Department of Disease and Stress Biology
[2] National Institute of Biotechnology and Genetic Engineering,undefined
来源：
Molecular Biotechnology | 2002年 / 20卷
关键词：
Geminivirus; satellite; whitefly; begomovirus; DNA β;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
DNA β is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation. Sequence comparisons of the two available DNA β sequences has identified a highly conserved region upstream of a predicted hairpin structure. Abutting primers designed to this conserved region allows PCR-mediated amplification of the full-length DNA β component from total nucleic acid extracts isolated from infected plants originating from a variety of geographically distinct sources and host plants.
引用
收藏
页码：315 / 318
页数：3
相关论文
共 50 条
  • [31] Detection of Verrucomicrobia in a pasture soil by PCR-mediated amplification of 16S rRNA genes
    O'Farrell, KA
    Janssen, PH
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1999, 65 (09) : 4280 - 4284
  • [32] A Multiplex PCR Assay Mediated by Universal Primers for the Detection of Adulterated Meat in Mutton
    Liu, Wanwan
    Wang, Xiaonan
    Tao, Jing
    Xi, Bangsheng
    Xue, Man
    Sun, Wanping
    JOURNAL OF FOOD PROTECTION, 2019, 82 (02) : 325 - 330
  • [33] RAPD-PCR amplification of soybean DNA using pairwise combinations of primers
    Correa, RX
    deBarros, EG
    Faleiro, FG
    Moreira, MA
    BRAZILIAN JOURNAL OF GENETICS, 1997, 20 (02): : 307 - 310
  • [34] Design and evaluation of PCR primers for amplification of four chloroplast DNA regions in plants
    Santos, Chiara
    Pereira, Filipe
    CONSERVATION GENETICS RESOURCES, 2017, 9 (01) : 9 - 12
  • [35] Design and evaluation of PCR primers for amplification of four chloroplast DNA regions in plants
    Chiara Santos
    Filipe Pereira
    Conservation Genetics Resources, 2017, 9 : 9 - 12
  • [36] Organelle DNA analysis of Solanum and Brassica somatic hybrids by PCR with ’universal primers’
    T. Bastia
    N. Scotti
    T. Cardi
    Theoretical and Applied Genetics, 2001, 102 : 1265 - 1272
  • [37] Organelle DNA analysis of Solanum and Brassica somatic hybrids by PCR with 'universal primers'
    Bastia, T
    Scotti, N
    Cardi, T
    THEORETICAL AND APPLIED GENETICS, 2001, 102 (08) : 1265 - 1272
  • [38] Comparison and critical evaluation of PCR-mediated methods to walk along the sequence of genomic DNA
    Yuki Tonooka
    Masahiro Fujishima
    Applied Microbiology and Biotechnology, 2009, 85 : 37 - 43
  • [39] Comparison and critical evaluation of PCR-mediated methods to walk along the sequence of genomic DNA
    Tonooka, Yuki
    Fujishima, Masahiro
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 85 (01) : 37 - 43
  • [40] Selection of universal primers for PCR quantification of total bacteria associated with fish fillets
    Lee, Juna-Lim
    Levin, Robert E.
    FOOD BIOTECHNOLOGY, 2006, 20 (03) : 275 - 285
12345