Universal primers for the PCR-mediated amplification of DNA βA molecule associated with some monopartite begomoviruses

被引：0
|
作者：
R. W. Briddon
S. E. Bull
S. Mansoor
I. Amin
P. G. Markham
机构：
[1] John Innes Centre,Department of Disease and Stress Biology
[2] National Institute of Biotechnology and Genetic Engineering,undefined
来源：
Molecular Biotechnology | 2002年 / 20卷
关键词：
Geminivirus; satellite; whitefly; begomovirus; DNA β;
D O I：
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中图分类号：
学科分类号：
摘要：
DNA β is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation. Sequence comparisons of the two available DNA β sequences has identified a highly conserved region upstream of a predicted hairpin structure. Abutting primers designed to this conserved region allows PCR-mediated amplification of the full-length DNA β component from total nucleic acid extracts isolated from infected plants originating from a variety of geographically distinct sources and host plants.
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页码：315 / 318
页数：3
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