Cytokeratin-19 associated with apoptosis and chemosensitivity in human cervical cancer cells

被引:0
|
作者
C.-C. Yuan
H.-C. Huang
L.-C. Tsai
H.-T. Ng
T.-S. Huang
机构
[1] National Yang-Ming University,Department of Obstetrics and Gynaecology, Veterans General Hospital (VGH)
[2] National Taiwan University,Taipei, and School of Medicine
[3] VGH-Taipei,Institute of Biochemistry, College of Medicine
[4] National Health Research Institutes,Department of Medical Research
来源
Apoptosis | 1997年 / 2卷
关键词
Apoptosis; cytokeratin; drug resistance;
D O I
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中图分类号
学科分类号
摘要
Cytokeratins are one group of intermediate filament proteins responsible for the integrity of cell structure, and have been recently reported to play a role in conferring a drug resistance phenotype. MAb Cx-99 is a monoclonal antibody exhibiting the specificity toward its corresponding antigen which was recently identified as the cytokeratin-19 protein. In the present study, we found that the level of cytokeratin-19 in cervical cancer cells could be decreased by incubation of cancer cells with MAb Cx-99. The reduction of cytokeratin-19 level had a killing effect on cervical carcinoma SIHA and HeLa S3 cell lines. The DNA ladder pattern, convoluted nuclei and blebbing morphology were observed with these cells after exposure to MAb Cx-99 for 72 h, suggesting that the cytotoxic mechanism of reduced cytokeratin-19 was mediated by induction of apoptosis. Moreover, the MAb Cx-99 treatment could increase the cytotoxicities of cancer chemotherapeutic agents such as cisplatin and vinblastine to both cervical carcinoma cell lines. The LD80 values were at least 15-fold reduced when cancer cells were treated with cisplatin or vinblastine in the presence of MAb Cx-99. These results suggest that the functional role of cytokeratin-19 was associated with the apoptosis prevention and drug resistance of cervical cancer cells.
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页码:101 / 105
页数:4
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