Modeling cardiac fibroblast heterogeneity from human pluripotent stem cell-derived epicardial cells

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作者
Ian Fernandes
Shunsuke Funakoshi
Homaira Hamidzada
Slava Epelman
Gordon Keller
机构
[1] University Health Network,McEwen Stem Cell Institute
[2] University of Toronto,Department of Medical Biophysics
[3] University Health Network,Princess Margaret Cancer Center
[4] University Health Network Toronto,Toronto General Hospital Research Institute
[5] Translational Biology and Engineering Program,Ted Rogers Centre for Heart Research
[6] University of Toronto,Department of Immunology
[7] University of Toronto,Department of Laboratory Medicine and Pathobiology
[8] University Health Networ,Peter Munk Cardiac Centre
[9] Kyoto University,Center for iPS Cell Research and Application
来源
Nature Communications | / 14卷
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摘要
Cardiac fibroblasts play an essential role in the development of the heart and are implicated in disease progression in the context of fibrosis and regeneration. Here, we establish a simple organoid culture platform using human pluripotent stem cell-derived epicardial cells and ventricular cardiomyocytes to study the development, maturation, and heterogeneity of cardiac fibroblasts under normal conditions and following treatment with pathological stimuli. We demonstrate that this system models the early interactions between epicardial cells and cardiomyocytes to generate a population of fibroblasts that recapitulates many aspects of fibroblast behavior in vivo, including changes associated with maturation and in response to pathological stimuli associated with cardiac injury. Using single cell transcriptomics, we show that the hPSC-derived organoid fibroblast population displays a high degree of heterogeneity that approximates the heterogeneity of populations in both the normal and diseased human heart. Additionally, we identify a unique subpopulation of fibroblasts possessing reparative features previously characterized in the hearts of model organisms. Taken together, our system recapitulates many aspects of human cardiac fibroblast specification, development, and maturation, providing a platform to investigate the role of these cells in human cardiovascular development and disease.
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