A study was conducted to investigate the relationship between urease activity and some physical, chemical, and microbiological properties of soils from central Iran. Inhibitory effects of Cr, Cd, and Pb on urease activity were also studied. Results indicated that no significant difference was observed between urease activity of field-moist and air-dried soils. Soil organic C and total N correlated highly significantly with urease activity, with r values of 0.899*** and 0.797***, respectively. There was also a significant correlation between urease activity and the number of bacteria grown on urea-agar media, with r value of 0.470*. A significant negative correlation (r =−0.492*) was observed between urease activity and electrical conductivity of saturation paste extracts. There were no significant correlations between urease activity and soil textural properties, pH, calcium carbonate equivalent, cation exchange capacity, and populations of soil bacteria on nutrient agar and population of soil fungi on potato dextrose agar. Both Cd and Pb inhibited urease activity to a similar extent and to a greater extent than did Cr.