Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells

被引：0
|
作者：
Sharron X. Lin
Barth Grant
David Hirsh
Frederick R. Maxfield
机构：
[1] Weill Medical College of Cornell University,Department of Biochemistry
[2] Columbia University,Department of Biochemistry and Molecular Biophysics
[3] College of Physicians and Surgeons,undefined
来源：
Nature Cell Biology | 2001年 / 3卷
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摘要：
RME-1 is an Eps15-homology (EH)-domain protein that was identified in a genetic screen for endocytosis genes in Caenorhabditis elegans. When expressed in a CHO cell line, the worm RME-1 protein and a mouse homologue are both associated with the endocytic recycling compartment. Here we show that expression of a dominant-negative construct with a point mutation near the EH domain results in redistribution of the endocytic recycling compartment and slowing down of transferrin receptor recycling. The delivery of a TGN38 chimaeric protein to the trans-Golgi network is also slowed down. The function of Rme-1 in endocytic recycling is evolutionarily conserved in metazoans as shown by the protein's properties in C. elegans.
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页码：567 / 572
页数：5
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