Microbacterium gilvum sp. nov., isolated from civet faeces

A novel aerobic, non-motile, Gram-positive, rod-shaped actinobacterium, designated YIM 100951T, was isolated from the faeces of civets (Viverra zibetha) living in the National Nature Protect Region in Selangor, Malaysia. Strain YIM 100951T shows high similarities with Microbacterium barkeri DSM 20145T (97.6 %), Microbacterium oryzae MB10T (97.3 %), Microbacterium lemovicicum ViU22T (97.1 %) and Microbacterium indicum BBH6T (97.0 %) based on their 16S rRNA genes. However, phylogenetic analysis showed that strain YIM 100951T formed a clade with Microbacterium halotolerans YIM 70130T (96.7 %), Microbacterium populi 10–107–8T (96.7 %) and Microbacterium sediminis YLB-01T (96.9 %). DNA–DNA hybridization was carried out between strains YIM 100951T and M. barkeri DSM 20145T, the result showed a value of 23.2 ± 4.5 %. In addition, some of the physiological, biochemical and chemotaxonomic characteristics of strain YIM 100951T are different from the closely related strains. Thus, we suggest that strain YIM 100951T represents a novel species of the genus Microbacterium, for which the name Microbacterium gilvum sp. nov. is proposed. The type strain is YIM 100951T (=DSM 26235T = CCTCC AB 2012971T).