RNA live imaging in microorganisms

被引：0
|
作者：
Müntjes K. [1 ]
Feldbrügge M. [1 ]
机构：
[1] Institut für Mikrobiologie, Cluster of Excellence on Plant Sciences, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, Düsseldorf
来源：
BIOspektrum | 2020年 / 26卷 / 1期
关键词：
D O I：
10.1007/s12268-020-1323-4
中图分类号：
学科分类号：
摘要：
RNA live imaging is a powerful technique to visualize the subcellular localization as well as highly dynamic transport of mRNAs in vivo. Established in eukaryotic microorganisms, it is now a wide-spread system used in bacteria, animals, and plants. The method is based on the binding of heterologous RNA-binding proteins to their cognate binding sites that are integrated into the mRNA of interest. Recent advances enable the visualization of transcriptional or translational sites as well as mRNA decay. © 2020, Die Autoren.
引用
收藏
页码：37 / 39
页数：2
相关论文
共 50 条
  • [31] Imaging LICA Oligo Rescue of RNA Toxicity in Live Mice with Myotonic Dystrophy
    Hu, Ningyan
    Antoury, Layal
    Baran, Timothy M.
    Rigo, Frank
    Bennett, C. Frank
    Foster, Thomas H.
    Wheeler, Thurman M.
    MOLECULAR THERAPY, 2017, 25 (05) : 120 - 120
  • [32] A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
    Esther Braselmann
    Aleksandra J. Wierzba
    Jacob T. Polaski
    Mikołaj Chromiński
    Zachariah E. Holmes
    Sheng-Ting Hung
    Dilara Batan
    Joshua R Wheeler
    Roy Parker
    Ralph Jimenez
    Dorota Gryko
    Robert T. Batey
    Amy E. Palmer
    Nature Chemical Biology, 2018, 14 : 964 - 971
  • [33] Intra-nuclear RNA trafficking: insights from live cell imaging
    Boulon, S
    Basyuk, E
    Blanchard, JM
    Bertrand, E
    Verheggen, C
    BIOCHIMIE, 2002, 84 (08) : 805 - 813
  • [34] CRISPR/Cas9: A New Tool for RNA Imaging in Live Cells
    Rau, Kristina
    Rentmeister, Andrea
    CHEMBIOCHEM, 2016, 17 (18) : 1682 - 1684
  • [35] Genetically Encoded Catalytic Hairpin Assembly for Sensitive RNA Imaging in Live Cells
    Mudiyanselage, Aruni P. K. K. Karunanayake
    Yu, Qikun
    Leon-Duque, Mark A.
    Zhao, Bin
    Wu, Rigumula
    You, Mingxu
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2018, 140 (28) : 8739 - 8745
  • [36] Doubly thiazole orange-labeled DNA for live cell RNA imaging
    Advanced Science Institute, RIKEN , Wako 351-0198, Japan
    Bull. Chem. Soc. Jpn., 1600, 1 (110-117):
  • [37] Imaging and Tracking RNA in Live Mammalian Cells via Fluorogenic Photoaffinity Labeling
    Quillin, Alexandria L.
    Karloff, Diane B.
    Ayele, Tewoderos M.
    Flores, Tatiana F.
    Chen, Gerry
    Mceachin, Zachary T.
    Valdez-Sinon, Arielle N.
    Heemstra, Jennifer M.
    ACS CHEMICAL BIOLOGY, 2025, 20 (03) : 707 - 720
  • [38] A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
    Braselmann, Esther
    Wierzba, Aleksandra J.
    Polaski, Jacob T.
    Chrominski, Mikolaj
    Holmes, Zachariah E.
    Hung, Sheng-Ting
    Batan, Dilara
    Wheeler, Joshua R.
    Parker, Roy
    Jimenez, Ralph
    Gryko, Dorota
    Batey, Robert T.
    Palmer, Amy E.
    NATURE CHEMICAL BIOLOGY, 2018, 14 (10) : 964 - +
  • [39] λN-GFP:: An RNA reporter system for live-cell imaging
    Daigle, Nathalie
    Ellenberg, Jan
    NATURE METHODS, 2007, 4 (08) : 633 - 636
  • [40] Doubly Thiazole Orange-Labeled DNA for Live Cell RNA Imaging
    Kubota, Takeshi
    Ikeda, Shuji
    Okamoto, Akimitsu
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 2009, 82 (01) : 110 - 117
12345