A toolkit for high-throughput, cross-species gene engineering in Drosophila

被引：0
|
作者：
Ejsmont R.K. [1 ]
Sarov M. [1 ]
Winkler S. [1 ]
Lipinski K.A. [1 ]
Tomancak P. [1 ]
机构：
[1] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden
来源：
Nature Methods | 2009年 / 6卷 / 6期
关键词：
D O I：
10.1038/nmeth.1334
中图分类号：
学科分类号：
摘要：
We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.
引用
收藏
页码：435 / 437
页数：2
相关论文
共 50 条
  • [21] High-throughput ethomics in large groups of Drosophila
    Branson K.
    Robie A.A.
    Bender J.
    Perona P.
    Dickinson M.H.
    Nature Methods, 2009, 6 (6) : 451 - 457
  • [22] A High-Throughput Method for Quantifying Drosophila Fecundity
    Gomez, Andreana
    Gonzalez, Sergio
    Oke, Ashwini
    Luo, Jiayu
    Duong, Johnny B.
    Esquerra, Raymond M.
    Zimmerman, Thomas
    Capponi, Sara
    Fung, Jennifer C.
    Nystul, Todd G.
    TOXICS, 2024, 12 (09)
  • [23] Correction: StreaMD: the toolkit for high-throughput molecular dynamics simulations
    Aleksandra Ivanova
    Olena Mokshyna
    Pavel Polishchuk
    Journal of Cheminformatics, 16 (1)
  • [24] Pyicos: a versatile toolkit for the analysis of high-throughput sequencing data
    Althammer, Sonja
    Gonzalez-Vallinas, Juan
    Ballare, Cecilia
    Beato, Miguel
    Eyras, Eduardo
    BIOINFORMATICS, 2011, 27 (24) : 3333 - 3340
  • [25] Drosophila mediopunctata microsatellites II: cross-species amplification in the tripunctata group and other Drosophila species
    Laborda, Prianda R.
    Klaczko, Louis B.
    de Souza, Anete P.
    CONSERVATION GENETICS RESOURCES, 2009, 1 (01) : 281 - 296
  • [26] Drosophila mediopunctata microsatellites II: cross-species amplification in the tripunctata group and other Drosophila species
    Prianda R. Laborda
    Louis B. Klaczko
    Anete P. de Souza
    Conservation Genetics Resources, 2009, 1
  • [27] Cross-species onocogenomics in cancer gene idenfification
    Peeper, Daniel
    Berns, Anton
    CELL, 2006, 125 (07) : 1230 - 1233
  • [28] Combinatorial gene libraries and high-throughput screening for rapid protein engineering.
    Hine, AV
    Hughes, MD
    Zhang, ZR
    Nagel, DA
    Ashraf, M
    Santos, AF
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2002, 224 : U252 - U252
  • [29] A high-throughput method for quantifying gene expression data from early Drosophila embryos
    Janssens, H
    Kosman, D
    Vanario-Alonso, CE
    Jaeger, J
    Samsonova, M
    Reinitz, J
    DEVELOPMENT GENES AND EVOLUTION, 2005, 215 (07) : 374 - 381
  • [30] A high-throughput method for quantifying gene expression data from early Drosophila embryos
    Hilde Janssens
    Dave Kosman
    Carlos E. Vanario-Alonso
    Johannes Jaeger
    Maria Samsonova
    John Reinitz
    Development Genes and Evolution, 2005, 215 : 374 - 381
12345