A toolkit for high-throughput, cross-species gene engineering in Drosophila

被引：0
|
作者：
Ejsmont R.K. [1 ]
Sarov M. [1 ]
Winkler S. [1 ]
Lipinski K.A. [1 ]
Tomancak P. [1 ]
机构：
[1] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden
来源：
Nature Methods | 2009年 / 6卷 / 6期
关键词：
D O I：
10.1038/nmeth.1334
中图分类号：
学科分类号：
摘要：
We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.
引用
收藏
页码：435 / 437
页数：2
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