Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

被引:0
|
作者
Sami Hocine
Pascal Raymond
Daniel Zenklusen
Jeffrey A Chao
Robert H Singer
机构
[1] Albert Einstein College of Medicine,Department of Anatomy and Structural Biology
[2] Université de Montréal,Department of Biochemistry
来源
Nature Methods | 2013年 / 10卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
Tagging of single transcripts with two fluorescent markers can be used to study many aspects of gene expression, including intrinsic noise in transcription or polymerase dynamics at a single gene.
引用
收藏
页码:119 / 121
页数:2
相关论文
共 50 条
  • [41] Single-molecule live-cell RNA imaging with CRISPR-Csm
    Xia, Chenglong
    Colognori, David
    Jiang, Xueyang Stephen
    Xu, Ke
    Doudna, Jennifer A.
    NATURE BIOTECHNOLOGY, 2025,
  • [42] Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae
    Patel, Heta P.
    Brouwer, Ineke
    Lenstra, Tineke L.
    STAR PROTOCOLS, 2021, 2 (03):
  • [43] RNA Trafficking between Membraneless Organelles at Single-Molecule Resolution in Live Cells
    Gao, Guoming
    Jalihal, Ameya P.
    Schmidt, Andreas
    Walter, Nils G.
    BIOPHYSICAL JOURNAL, 2020, 118 (03) : 467A - 468A
  • [44] Analysis of Phase Heterogeneity in Lipid Membranes Using Single-Molecule Tracking in Live Cells
    Park, Jiseong
    Ahn, Yongdeok
    Lee, Wonhee John
    Jin, Siwoo
    Jeong, Sejoo
    Kim, Jaeyong
    Lee, Young-Sam
    Lee, Jong-Chan
    Seo, Daeha
    ANALYTICAL CHEMISTRY, 2023, 95 (43) : 15924 - 15932
  • [45] Analysis of Phase Heterogeneity in Lipid Membranes Using Single-Molecule Tracking in Live Cells
    Park, Jiseong
    Ahn, Yongdeok
    Lee, Wonhee John
    Jin, Siwoo
    Jeong, Sejoo
    Kim, Jaeyong
    Lee, Young-Sam
    Lee, Jong-Chan
    Seo, Daeha
    ANALYTICAL CHEMISTRY, 2023,
  • [46] Analysis of Phase Heterogeneity in Lipid Membranes Using Single-Molecule Tracking in Live Cells
    Park, Jiseong
    Ahn, Yongdeok
    Lee, Wonhee John
    Jin, Siwoo
    Jeong, Sejoo
    Kim, Jaeyong
    Lee, Young-Sam
    Lee, Jong-Chan
    Seo, Daeha
    ANALYTICAL CHEMISTRY, 2023,
  • [47] Partitioning of RNA Polymerase Activity in Live Escherichia coli from Analysis of Single-Molecule Diffusive Trajectories
    Bakshi, Somenath
    Dalrymple, Renee M.
    Li, Wenting
    Choi, Heejun
    Weisshaar, James C.
    BIOPHYSICAL JOURNAL, 2013, 105 (12) : 2676 - 2686
  • [48] Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells
    Li, Jieru
    Dong, Ankun
    Saydaminova, Kamola
    Chang, Hill
    Wang, Guanshi
    Ochiai, Hiroshi
    Yamamoto, Takashi
    Pertsinidis, Alexandros
    CELL, 2019, 178 (02) : 491 - +
  • [49] Two-color in vivo Single mRNA Counting in Yeast for Measuring Intrinsic Noise
    Hocine, S.
    Zenklusen, D.
    Chao, J. A.
    Singer, R. H.
    MOLECULAR BIOLOGY OF THE CELL, 2011, 22
  • [50] Two-Color Single-Molecule Blinking Ratiometricity: A Functional Super-Resolution Imaging Approach for Resolving Lysosomal pH and Dynamics
    Qiao, Qinglong
    Yin, Wenting
    Wu, Xia
    Wu, Shaowei
    Ruan, Yiyan
    Xu, Ning
    Li, Jin
    Wu, Zhong-Shuai
    Liu, Xiaogang
    Xu, Zhaochao
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2025,