Poly A RNA status and expression of milli (m) and micro (μ) calpains in skeletal muscle of duchenne muscular dystrophy patients

被引：0
|
作者：
Mangat H. [1 ]
Hussain T. [2 ]
Sundaram C. [3 ]
Anandaraj M.P.J.S. [1 ]
机构：
[1] Department of Molecular Biology and Biochemistry, Institute of Genetics, Hospital for Genetic Diseases, Begumpet
[2] Centre for DNA Fingerprinting and Diagnostics, Nacharam, Hyderabad
[3] Department of Pathology, Nizams Institute of Medical Sciences, Punjagutta, Hyderabad
来源：
Indian Journal of Clinical Biochemistry | 2002年 / 17卷 / 2期
关键词：
Calpain; DMD; in vitro translation; Poly A RNA; Protein synthesis; Skeletal muscle;
D O I：
10.1007/BF02867975
中图分类号：
学科分类号：
摘要：
Poly A RNA status and its in vitro translation in a rabbit reticulolysate cell free system, were assessed in the skeletal muscle of young boys with Duchenne muscular dystrophy. Compared to normals there is a significant 48% increase in poly A RNA content of dystrophic muscle and its translatability was increased by 56% based on 35S methionine incorporation into total protein systhesised. Immunoprecipitation of the translated products with monospecific antibodies showed that there is a 2.6 fold and a 2 fold increase in m and μ calpains respectively. This underlines the importance of both synthetic and degenerative activities in the early pathology in DMD muscle.
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页码：74 / 79
页数：5
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