PAR 1-type Thrombin Receptors are Involved in Thrombin-induced Calcium Signaling in Human Meningioma Cells

被引:0
|
作者
Roland Kaufmann
Stephan Patt
Robert Kraft
Michael Zieger
Peter Henklein
Gunter Neupert
Götz Nowak
机构
[1] Friedrich Schiller University Jena,Research Unit ‘Pharmacological Hemostaseology’
[2] Friedrich Schiller University Jena,Institute of Pathology (Neuropathology)
[3] Humboldt University,Institute of Biochemistry
来源
Journal of Neuro-Oncology | 1999年 / 42卷
关键词
proteinase-activated receptor 1; PAR 1; meningioma cells; thrombin; calcium signaling;
D O I
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中图分类号
学科分类号
摘要
Thrombin is known to play a role as regulator in tumor spreading and tumor growth. Proteinase-activated receptor 1 (PAR 1)-type thrombin receptors were identified in different cancer cells including human glioblastoma cells. Thus a function of PAR 1 in brain tumors may be suggested. In this study, the presence of PAR 1-type thrombin receptors was investigated in primary cell cultures established from operated human meningiomas from two 59- and 79-year-old women. Characterization of PAR 1 on binding level was performed using immunofluorescence studies with the monoclonal anti-PAR 1 antibody Mab 61-1 directed against a domain in the NH2-terminus of PAR 1. These binding sites constitute functional thrombin receptors that are involved in thrombin-induced signaling in human meningioma cells as demonstrated by investigation of alpha-thrombin- and PAR 1-activating hexapeptide (TRAP-6)-induced [Ca2+]i mobilization. To our knowledge, this is the first report demonstrating thrombin-induced intracellular signaling in human meningioma cells mediated by the PAR 1-type thrombin receptor.
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页码:131 / 136
页数:5
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