Rapid detection of food-borne Listeria monocytogenes by real-time quantitative loop-mediated isothermal amplification

被引：0
|
作者：
Xiaoxiao Shan
Yaoqi Zhang
Zhigang Zhang
Miaorui Chen
Yongyu Su
Yingna Yuan
M. Jahangir Alam
He Yan
Lei Shi
机构：
[1] South China University of Technology,College of Light Industry and Food Sciences
[2] South China University of Technology,School of Bioscience and Bioengineering
[3] Xiamen Yinxiang Group Co.,State Key Laboratory of Food Safety Technology for Meat Products
[4] Ltd.,College of Landscape and Art
[5] Jiangxi Agricultural University,undefined
[6] Texas Commission on Environmental Quality,undefined
来源：
Food Science and Biotechnology | 2012年 / 21卷
关键词：
loop-mediated isothermal amplication (LAMP); real-time turbidimeter; quantitative;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The purpose of this study was to develop a real-time quantitative loop-mediated isothermal amplication (LAMP) method for the rapid, sensitive, and convenient detection of Listeria monocytogenes in food. The LAMP method could amplify the hlyA gene of L. monocytogenes successfully at 63°C with a loopamp real-time turbidimeter. The detection limits of the LAMP for hlyA gene were 6 colony forming units (CFU)/tube. A standard curve was generated for L. monocytogenes LAMP by plotting the graph based different log CFU values of L. monocytogenes and time of positivity through real-time monitoring of the amplication. Then, the LAMP method was employed to test 94 retail food samples effectively. Sensitivity in detection of L. monocytogenes by the LAMP was higher than that of PCR and none of the conventional methodpositive samples was missed by the LAMP method.
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页码：101 / 106
页数：5
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