Cloning of two β-xylanase-encoding genes from Aspergillus niger and their expression in Saccharomyces cerevisiae

被引：0
|
作者：
M. Luttig
I.S. Pretorius
W.H. van Zyl
机构：
[1] University of Stellenbosch,Department of Microbiology
来源：
Biotechnology Letters | 1997年 / 19卷
关键词：
Xylanase Activity; Xylanase Gene; Feruloyl Esterase; Xylanolytic Enzyme; ADH2 Promoter;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two endo-β-1,4-xylanase-encoding genes were amplified from Aspergillus niger ATCC 90196 mRNA, inserted between the yeast ADH2 promoter and terminator sequences (genes designated XYN4 and XYN5) and expressed in Saccharomyces cerevisiae. The nucleotide sequences of the XYN4 and XYN5 genes revealed that both genes encode 211-amino acid proteins that are 92% identical to each other. Both the Xyn4 and Xyn5 enzymes have pH and temperature optima of pH 4 and 60°C, respectively. Autoselective S. cerevisiae strains were developed that allowed β-xylanase production and secretion in complex medium.
引用
收藏
页码：411 / 415
页数：4
相关论文
共 50 条
  • [31] Molecular Cloning and Overexpression of an Endo-β-1,4-xylanase Gene from Aspergillus niger in Industrial Saccharomyces cerevisiae YS2 Strain
    Baoyu Tian
    Yan Xu
    Wanling Cai
    Qingeng Huang
    Yuanyuan Gao
    Xin Li
    Jianzhong Huang
    Applied Biochemistry and Biotechnology, 2013, 170 : 320 - 328
  • [32] Molecular Cloning and Overexpression of an Endo-β-1,4-xylanase Gene from Aspergillus niger in Industrial Saccharomyces cerevisiae YS2 Strain
    Tian, Baoyu
    Xu, Yan
    Cai, Wanling
    Huang, Qingeng
    Gao, Yuanyuan
    Li, Xin
    Huang, Jianzhong
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2013, 170 (02) : 320 - 328
  • [33] Molecular cloning, sequence analysis and expression of a GHF 43 xylanase from Aspergillus niger in Escherichia coli
    Zhou, Chen-Yan
    Wang, Yong-Tao
    Zhu, Tie-Chui
    Fu, Guan-Hua
    Wang, Dan-Dan
    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2014, 60 (06): : 234 - 240
  • [34] Cloning, Expression, and Characterization of an GHF 11 Xylanase from Aspergillus niger XZ-3S
    Fu, Guanhua
    Wang, Yongtao
    Wang, Dandan
    Zhou, Chenyan
    INDIAN JOURNAL OF MICROBIOLOGY, 2012, 52 (04) : 682 - 688
  • [35] Cloning, Expression, and Characterization of an GHF 11 Xylanase from Aspergillus niger XZ-3S
    Guanhua Fu
    Yongtao Wang
    Dandan Wang
    Chenyan Zhou
    Indian Journal of Microbiology, 2012, 52 : 682 - 688
  • [36] Functional expression of amine oxidase from Aspergillus niger (AO-I) in Saccharomyces cerevisiae
    Kateřina Kolaříková
    Petr Galuszka
    Iva Sedlářová
    Marek Šebela
    Ivo Frébort
    Molecular Biology Reports, 2009, 36 : 13 - 20
  • [37] Functional expression of amine oxidase from Aspergillus niger (AO-I) in Saccharomyces cerevisiae
    Kolarikova, Katerina
    Galuszka, Petr
    Sedlarova, Iva
    Sebela, Marek
    Frebort, Ivo
    MOLECULAR BIOLOGY REPORTS, 2009, 36 (01) : 13 - 20
  • [38] EXPRESSION OF THE ASPERGILLUS-NIGER GLUCOSE-OXIDASE GENE IN ASPERGILLUS-NIGER, ASPERGILLUS-NIDULANS AND SACCHAROMYCES-CEREVISIAE
    WHITTINGTON, H
    KERRYWILLIAMS, S
    BIDGOOD, K
    DODSWORTH, N
    PEBERDY, J
    DOBSON, M
    HINCHLIFFE, E
    BALLANCE, DJ
    CURRENT GENETICS, 1990, 18 (06) : 531 - 536
  • [39] CLONING, CHARACTERIZATION AND EXPRESSION OF PEPF, A GENE ENCODING A SERINE CARBOXYPEPTIDASE FROM ASPERGILLUS-NIGER
    VANDENHOMBERGH, JPTW
    JARAI, G
    BUXTON, FP
    VISSER, J
    GENE, 1994, 151 (1-2) : 73 - 79
  • [40] EFFECTS OF DISRUPTION OF XYLANASE-ENCODING GENES ON THE XYLANOLYTIC SYSTEM OF STREPTOMYCES-LIVIDANS
    ARHIN, FF
    SHARECK, F
    KLUEPFEL, D
    MOROSOLI, R
    JOURNAL OF BACTERIOLOGY, 1994, 176 (16) : 4924 - 4930
12345