Toward a multicolor chromosome bar code for the entire human karyotype by fluorescence in situ hybridization

被引:0
|
作者
S. Müller
M. Rocchi
M. A. Ferguson-Smith
J. Wienberg
机构
[1] Department of Pathology,
[2] Cambridge University,undefined
[3] Tennis Court Road,undefined
[4] Cambridge CB2 1QP,undefined
[5] UK Tel.: +44-1223-333725; Fax: +44-1223-333346; e-mail: jw@mole.bio.cam.ac.uk,undefined
[6] Istituto di Genetica,undefined
[7] University of Bari,undefined
[8] I-70126 Bari,undefined
[9] Italy,undefined
来源
Human Genetics | 1997年 / 100卷
关键词
Banding Pattern; Chromosome Rearrangement; Cell Hybrid; Hybridization Experiment; Distinct Signal;
D O I
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中图分类号
学科分类号
摘要
A colored banding pattern for human chromosomes is described that distinguishes each chromosome in a single fluorescence in situ hybridization with a set of subregional DNA probes. Alu/polymerase chain reaction products of various human/rodent somatic cell hybrids (fragment hybrids) were pooled into two probe sets that were labeled differentially and detected by red and green fluorescence. Chromosome regions hybridized by DNA present in both pools appeared yellow. The result was a multi-color set of 110 distinct signals per haploid chromosome set for the human karyotype. Each individual chromosome showed a unique sequence of signals, a result termed the “chromosome bar code”. The reproducibility of the hybridization pattern in various labeling and hybridization experiments was analyzed by computer densitometry. We have applied the chromosome bar code both in diagnostic cytogenetics and in genome studies. The approach allows the rapid identification of chromosomes and chromosome rearrangements. Although not yet showing the resolution of classical banding patterns, the present experiments demonstrate various applications in which the present multi-color bar code can significantly add to the spectrum of cytogenetic techniques.
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页码:271 / 278
页数:7
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