Neurotoxicity evoked by N-methyl-d-aspartate in the organotypic static slice cultures of rat cerebral cortices: effect of GABAA receptor activation

被引:0
|
作者
Makoto Yoshikawa
S. Goto
Akira Okamura
Tadashi Hamasaki
Yukitaka Ushio
机构
[1] Laboratory for Cerebrovascular Disorders and Neuroscience,
[2] Department of Neurosurgery,undefined
[3] Kumamoto University Medical School,undefined
[4] Kumamoto 860,undefined
[5] Japan Tel.: 81-96-373-5220; Fax: 81-96-372-6140,undefined
来源
Acta Neuropathologica | 1998年 / 95卷
关键词
Key words Cerebral cortex; Organotypic slice culture; N-methyl-d-aspartate; Muscimol; Neuronal injury;
D O I
暂无
中图分类号
学科分类号
摘要
We investigated the neurotoxicity evoked by N-methyl-d-aspartate (NMDA) receptor stimulation in the organotypic static slice cultures of rat cerebral cortices. We also examined whether the γ-aminobutyric acid (GABA)A receptor agonist muscimol has a protective effect on the NMDA-mediated neurotoxicity in this culture system. NMDA-mediated cytotoxicity was evaluated histologically and quantified by the measurement of lactate dehydrogenase (LDH) release into the culture medium. There was an NMDA-induced, dose-dependent leakage of LDH release and neuronal cell death, which were not attenuated by muscimol treatment. The results suggested that NMDA neurotoxicity is reproduced in the organotypic culture, and that GABAA receptor activation exerted no protective action against the NMDA cytotoxicity.
引用
收藏
页码:592 / 596
页数:4
相关论文
共 50 条
  • [21] Effect of acidotic challenges on local depolarizations evoked by N-methyl-D-aspartate in the rat striatum
    Urenjak, J
    Zilkha, E
    Gotoh, M
    Obrenovitch, TP
    LIFE SCIENCES, 1997, 61 (05) : 523 - 535
  • [22] Effect of N-methyl-D-aspartate receptor blockade on caspase activation and neuronal death in the developing rat cerebellum
    Alavez, Silvestre
    Blancas, Sugela
    Moran, Julio
    NEUROSCIENCE LETTERS, 2006, 404 (1-2) : 176 - 181
  • [23] INVOLVEMENT OF N-METHYL-D-ASPARTATE RECEPTORS IN EPILEPTIFORM BURSTING IN THE RAT HIPPOCAMPAL SLICE
    DINGLEDINE, R
    HYNES, MA
    KING, GL
    JOURNAL OF PHYSIOLOGY-LONDON, 1986, 380 : 175 - 189
  • [24] RECEPTOR-LINKED IONIC CHANNELS MEDIATE N-METHYL-D-ASPARTATE NEUROTOXICITY IN RAT CEREBELLAR SLICES
    GARTHWAITE, G
    GARTHWAITE, J
    NEUROSCIENCE LETTERS, 1987, 83 (03) : 241 - 246
  • [25] Effects of adenosine and γ-aminobutyric acid a receptor antagonists on N-methyl-D-aspartate induced neurotoxicity in the rat hippocampus
    Robledo, P
    Ursu, G
    Mahy, N
    HIPPOCAMPUS, 1999, 9 (05) : 527 - 533
  • [26] Anti-neurodegenerative effects of tacrine on N-methyl-D-aspartate (NMDA)-induced neuronal cell death in organotypic hippocampal slice cultures
    Inada, Chikako
    Yamada, Marina
    Zhao, Qi
    Matsumoto, Kinzo
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2011, 115 : 247P - 247P
  • [27] N-methyl-D-aspartate receptor activation in human cerebral endothelium promotes intracellular oxidant stress
    Sharp, CD
    Houghton, J
    Elrod, JW
    Warren, A
    Jackson, TH
    Jawahar, A
    Nanda, A
    Minagar, A
    Alexander, JS
    AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2005, 288 (04): : H1893 - H1899
  • [28] Oxygen and Glucose Deprivation in an Organotypic Hippocampal Slice Model of the Developing Rat Brain: The Effects on N-Methyl-D-Aspartate Subunit Composition
    Wise-Faberowski, Lisa
    Robinson, Prairie Neeley
    Rich, Sarah
    Warner, David S.
    ANESTHESIA AND ANALGESIA, 2009, 109 (01): : 205 - 210
  • [29] A N-METHYL-D-ASPARTATE RECEPTOR-MEDIATED NEUROTOXIC EFFECT OF ASPARTATE-BASED HYDROXAMATE COMPOUNDS IN RAT PRIMARY NEURONAL CULTURES
    LOCKHART, BP
    HAMEDISANGSARI, F
    VIGNON, J
    PRIVAT, A
    VILA, J
    BRAIN RESEARCH, 1995, 670 (02) : 313 - 316
  • [30] The vasodilatory effect of ketamine is independent of the N-methyl-D-aspartate receptor: lack of functional N-methyl-D-aspartate receptors in rat mesenteric artery smooth muscle
    Noh, Hyun J.
    Bae, Young M.
    Park, Seung H.
    Kim, Jae G.
    Kim, Bokyung
    Kim, Yoon S.
    Kim, Seong H.
    Cho, Sung-Il
    Woo, Nam S.
    EUROPEAN JOURNAL OF ANAESTHESIOLOGY, 2009, 26 (08) : 676 - 682