New fructan — the O-specific polysaccharide from Escherichia coli O92

被引:0
|
作者
S. N. Senchenkova
Guangnan Xu
Yanfen Qi
A. V. Perepelov
A. S. Shashkov
Bin Liu
Yu. A. Knirel
机构
[1] Russian Academy of Sciences,N. D. Zelinsky Institute of Organic Chemistry
[2] Nankai University,Institute of Biological Sciences and Biotechnology, Tianjin Economic
来源
Russian Chemical Bulletin | 2017年 / 66卷
关键词
structure of the O-specific polysaccharide; O-antigen gene cluster; lipopolysaccharide; fructan;
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摘要
The structure of the O-specific polysaccharide (O-antigen) from Escherichia coli O92 was established. The acid-labile polysaccharide was produced by O-deacylation of the lipopolysaccharide under mild alkaline conditions. The methylation analysis combined with 1D and 2D 1H and 13C NMR spectroscopy demonstrated that the O-specific polysaccharide chain of the lipopolysaccharide is a linear fructan composed of mono-О-acetylated disaccharide repeating units having the structure →1)-β-d-Fruf3Ac-(2→3)-β-d-Fruf-(2→. To the best of our knowledge, this fructan has been hitherto unknown in bacteria.
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页码:1304 / 1307
页数:3
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