Methods for microbial DNA extraction from soil for PCR amplification

被引：199
|
作者：
Yeates C. [1 ]
Gillings M.R. [1 ]
Davison A.D. [1 ]
Altavilla N. [1 ]
Veal D.A. [1 ]
机构：
[1] Key Centre for Biodiversity and Bioresources, School of Biological Sciences, Macquarie University, Sydney
来源：
Biological Procedures Online | 1998年 / 1卷 / 1期
关键词：
Humic Acid; Internal Transcribe Space; Environmental Microbiology; nifH Gene; Biological Procedure;
D O I：
10.1251/bpo6
中图分类号：
学科分类号：
摘要：
Amplification of DNA from soil is often inhibited by co-purified contaminants. A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types (1). DNA is also suitable for PCR amplification using various DNA targets. DNA was extracted from l00g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation. This method was compared to other DNA extraction methods with regard to DNA purity and size. ©1998 Biological Procedures Online. All rights reserved.
引用
收藏
页码：40 / 47
页数：7
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