Combinatorial immunotherapy with gemcitabine and ex vivo-expanded NK cells induces anti-tumor effects in pancreatic cancer

被引:0
|
作者
Eun-Kyoung Koh
Hong-Rae Lee
Woo-Chang Son
Ga-Young Park
Juhee Kim
Jae-Ho Bae
You-Soo Park
机构
[1] Dongnam Institute of Radiological and Medical Sciences,Department of Research Center
[2] Pusan National University School of Medicine,Department of Biochemistry
来源
Scientific Reports | / 13卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
Pancreatic cancer is difficult to diagnose at the initial stage and is often discovered after metastasis to nearby organs. Gemcitabine is currently used as a standard treatment for pancreatic cancer. However, since chemotherapy for pancreatic cancer has not yet reached satisfactory therapeutic results, adjuvant chemotherapy methods are attempted. It can be expected that combining immune cell therapy with existing anticancer drug combination treatment will prevent cancer recurrence and increase survival rates. We isolated natural killer (NK) cells and co-cultured them with strongly activated autologous peripheral blood mononuclear cells (PBMCs) as feeder cells, activated using CD3 antibody, IFN-r, IL-2, and γ-radiation. NK cells expanded in this method showed greater cytotoxicity than resting NK cells, when co-cultured with pancreatic cancer cell lines. Tumor growth was effectively inhibited in a pancreatic cancer mouse xenograft model. Therapeutic efficacy was increased by using gemcitabine and erlotinib in combination. These findings suggest that NK cells cultured by the method proposed here have excellent anti-tumor activity. We demonstrate that activated NK cells can efficiently inhibit pancreatic tumors when used in combination with gemcitabine-based therapy.
引用
收藏
相关论文
共 50 条
  • [21] Efforts to improve the anti-tumor effect of gemcitabine in human pancreatic cancer
    Nakamori, Shoji
    Kashiwazaki, Masaki
    Ikenaga, Masakazu
    Miyazaki, Michihiko
    Hirao, Motohiro
    Fujitani, Kazumasa
    Mishima, Hideyuki
    Tsujinaka, Toshimasa
    Nakahira, Shin
    Tsujie, Masanori
    Takeda, Yutaka
    Monden, Morito
    PANCREAS, 2008, 37 (01) : 111 - 112
  • [22] The anti-canine distemper virus activities of ex vivo-expanded canine natural killer cells
    Park, Ji-Yun
    Shin, Dong-Jun
    Lee, Soo-Hyeon
    Lee, Je-Jung
    Suh, Guk-Hyun
    Cho, Duck
    Kim, Sang-Ki
    VETERINARY MICROBIOLOGY, 2015, 176 (3-4) : 239 - 249
  • [23] Synergistic anti-tumor effects of liraglutide with metformin on pancreatic cancer cells
    Lu, Ran
    Yang, Jin
    Wei, Rui
    Ke, Jing
    Tian, Qing
    Yu, Fei
    Liu, Junling
    Zhang, Jingjing
    Hong, Tianpei
    PLOS ONE, 2018, 13 (06):
  • [24] Cytotoxic effects of γδ T cells expanded ex vivo by a third generation bisphosphonate for cancer immunotherapy
    Sato, K
    Kimura, S
    Segawa, H
    Yokota, A
    Matsumoto, S
    Kuroda, J
    Nogawa, M
    Yuasa, T
    Kiyono, Y
    Wada, H
    Maekawa, T
    INTERNATIONAL JOURNAL OF CANCER, 2005, 116 (01) : 94 - 99
  • [25] NON-CLONAL CHROMOSOME ABERRATIONS IN EX VIVO EXPANDED NK CELLS APPLIED FOR IMMUNOTHERAPY
    Li, Yulong
    Huang, Zhoufeng
    Zhang, Lei
    Wu, Chengye
    Cheng, Wei
    Shang, Baojun
    Chen, Mingyi
    Murphy, Willaim
    Sun, Kai
    INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, 2017, 39 : 67 - 67
  • [26] Characterizing the anti-tumor function of adoptively transferred NK cells in vivo
    Hollie J. Pegram
    Nicole M. Haynes
    Mark J. Smyth
    Michael H. Kershaw
    Phillip K. Darcy
    Cancer Immunology, Immunotherapy, 2010, 59 : 1235 - 1246
  • [27] Characterizing the anti-tumor function of adoptively transferred NK cells in vivo
    Pegram, Hollie J.
    Haynes, Nicole M.
    Smyth, Mark J.
    Kershaw, Michael H.
    Darcy, Phillip K.
    CANCER IMMUNOLOGY IMMUNOTHERAPY, 2010, 59 (08) : 1235 - 1246
  • [28] Vitamin D treatment induces in vitro and ex vivo transcriptomic changes indicating anti-tumor effects
    Vaughan-Shaw, Peter G.
    Blackmur, James P.
    Grimes, Graeme
    Ooi, Li-Yin
    Ochocka-Fox, Anna M.
    Dunbar, Karen
    von Kriegsheim, Alex
    Rajasekaran, Vidya
    Timofeeva, Maria
    Walker, Marion
    Svinti, Victoria
    Din, Farhat V. N.
    Farrington, Susan M.
    Dunlop, Malcolm G.
    FASEB JOURNAL, 2022, 36 (01):
  • [29] Neuroprotective effects of ex vivo-expanded regulatory T cells on trimethyltin-induced neurodegeneration in mice
    Park, Seon-Young
    Yang, HyeJin
    Ye, Minsook
    Liu, Xiao
    Shim, Insop
    Chang, Young-Tae
    Bae, Hyunsu
    JOURNAL OF NEUROINFLAMMATION, 2022, 19 (01)
  • [30] Adoptive Immunotherapy of Human Gastric Cancer with Ex Vivo Expanded T Cells
    Kim, Yeon Jin
    Lim, Jaeseung
    Kang, Jong Soon
    Kim, Hwan Mook
    Lee, Hong Kyung
    Ryu, Hwa Sun
    Kim, Jee Youn
    Hong, Jin Tae
    Kim, Youngsoo
    Han, Sang-Bae
    ARCHIVES OF PHARMACAL RESEARCH, 2010, 33 (11) : 1789 - 1795