Pathway engineering of Saccharomyces cerevisiae for efficient lycopene production

被引:0
|
作者
Xian Xu
Jie Liu
Yongling Lu
Haiquan Lan
Liqing Tian
Zhidong Zhang
Chengjia Xie
Ling Jiang
机构
[1] Nanjing Normal University,School of Food Science and Pharmaceutical Engineering
[2] Nanjing Tech University,State Key Laboratory of Materials
[3] Xinjiang Academy of Agricultural Sciences,Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering
[4] Yangzhou Polytechnic Institute,Institute of Microbiology
[5] Nanjing Tech University,School of Chemical Engineering
来源
Bioprocess and Biosystems Engineering | 2021年 / 44卷
关键词
Lycopene; Chassis; Fatty acids; Constitutive promoter;
D O I
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中图分类号
学科分类号
摘要
To construct a Saccharomyces cerevisiae strain for efficient lycopene production, we used a pathway engineering strategy based on expression modules comprising fusion proteins and a strong constitutive promoter. The two recombinant plasmids pEBI encoding the fusion genes with an inducible promoter, as well as pIETB with a constitutive promoter and terminator were introduced into S. cerevisiae YPH499 and BY4741 to obtain the four recombinant strains ypEBI, ypIETB, byEBI and byIETB. The lycopene production and the transcription levels of key genes were higher in the BY4741 chassis than in YPH499. Accordingly, the content of total and unsaturated fatty acids was also higher in BY4741, which also exhibited a decrease of glucose, increase of trehalose, increase of metabolite in citrate cycle, and low levels of amino acids. These changes rerouted metabolic fluxes toward lycopene synthesis, indicating that the BY4741 chassis was more suitable for lycopene synthesis. The lycopene content of bpIETB in SG-Leu medium supplemented with 100 mg/L of linolenic acid reached 10.12 mg/g dry cell weight (DCW), which was 85.7% higher than without the addition of unsaturated fatty acids. The constitutive promoter expression strategy employed in this study achieved efficient lycopene synthesis in S. cerevisiae, and the strain bpIETB was obtained a suitable chassis host for lycopene production, which provides a basis for further optimization of lycopene production in artificial synthetic cells and a reference for the multi-enzyme synthesis of other similar complex terpenoids.
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页码:1033 / 1047
页数:14
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