Callus induction and somatic embryogenesis of Phalaenopsis

被引：0
|
作者：
Y. Ishii
T. Takamura
M. Goi
M. Tanaka
机构：
[1] Department of Horticulture,
[2] Faculty of Agriculture,undefined
[3] Kagawa University,undefined
[4] Miki-cho,undefined
[5] Kagawa 761-0795,undefined
[6] Japan,undefined
来源：
Plant Cell Reports | 1998年 / 17卷
关键词：
Key wordsPhalaenopsis; Protocorm-like bodies; Callus induction; Somatic embryogenesis;
D O I：
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中图分类号：
学科分类号：
摘要：
Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer `Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g ⋅ l–1. Medium containing 200 ml ⋅ l–1 coconut water together with 40 g ⋅ l–1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.
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页码：446 / 450
页数：4
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